Date of Award

Winter 2-2022

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Molecular Science and Nanotechnology

First Advisor

David Mills

Abstract

Magnetic Halloysite Nanotubes can be used in multiple ways in combating growth of osteosarcoma. The adsorption ability of the inner and outer surfaces of halloysite nanotubes allows for attachment of different molecules, including anti-cancer drugs, polymer layering for controlled drug release, and magnetic coating. This magnetic coating would allow for cell membrane disruption, hyperthermia, and for targeting of specific tissue. In this project, the goal was to create these magnetic nanotubes and test their cytotoxicity against stem cells and osteosarcoma cells.

To create these magnetic nanotubes, we used electrodeposition to adsorb ferrous oxide nanoparticles (FeNPs) onto halloysite nanotubes (HNTs) to create iron-coated halloysite nanotubes (FeHNTs). These FeHNTs were then imaged using a field emission scanning electron microscope (FESEM) and underwent elemental analysis, using the energy dispersive X-ray spectroscopy (EDS), to ensure placement of the FeNPs onto the HNTs.

The FeHNTs were then tested for their cytotoxicity and cell differentiation potential against human adipose-derived mesenchymal stem cells (hASCs) and mouse osteosarcoma cells. The viability of both cell lines was compared. Next, the proliferation capability, the potential tissue formation, and the potential ossification of hASCs was compared with increasing concentrations of FeHNTs.

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